Thread: Animal Cell Culture Report of Myostatin HMP

This thread provides the background of Myostatin HMP, explanations of current production, and the brief review of research that has been conducted at Labpe Peptide.


Background



Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a protein that in humans is encoded by the MSTN gene. It isproduced primarily in skeletal muscle cells, circulates in the blood and acts on muscle tissue.


The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide acting as a negative regulator of myostatin. Recombinant human Myostatin Propeptide is a non-glycosylated protein, containing 244 amino acids, with a molecular weight of 27.8 kDa.


Below is some research that has been done those are generally available online as well.


From: Regulation of myostatin activity and muscle growth


Transgene Mice:


DNAs encoding a truncated form of the murine myostatin propeptide (amino acids 1-267), were cloned into the MDAF2 vector containing the myosin light chain promoter and 1/3 enhancer and simian virus 40 processing sites




That is , the gene of the mice have already been programmed. This is not a result from injection of Myostatin HMP.


Current Production of Myostatin Propeptide



Is the current Myostatin HMP which is sold at around $100 per mg the real Myostatin Propeptide? 98% yes and 2% no.


As a manufacturer of Myostatin, we are always asked the question, is the Myostatin tagged?


The answer is yes and our Myostatin is tagged.


What is the tag?


The tags are chains/pile of amino acids/proteins, it is foreign protein that will cause immunization reaction in most cases. Search immunization reactions for more details.


Then, are they all fake or bunk?


Well, let?s look at the Labpe Peptide?s Rat Research


Warning: The research contains killing of animals, it is for research purpose only, please do not repeat the experiment if you are not a research scientist. All the experiments have to be done in facilities that abide by laws and do not produce any pollution to the environment.


Group A: One rat was given Myostatin HMP（1mg/vial）www.labpe.com with 300μg (300 mcg) every three days for six injections, and another was given the same amount every week (7 Days) for five weeks.


Group B: The two rats in this group were used as negative controls, injected with the same amount of physiological saline with the same respective frequency.


2．Experiment process



2.1 Rats Selecting



According to the plan, we selected, grouped and raised male rats with the weight of 150?10g.




2.2 Pre-processing of samples



Infused 2ml physiological saline in a vial of LABPE made Myostatin HMP(1mg/vial)，shaking till dissolution，and got the processed Myostatin solution with the concentration of 500μg/ml.




2.3 Injecting



Group A: the two rats were respectively injected 0.6ml Myostatin solution(500μg/ml).




2.4Comparison after Shaving



2.4.1Two weeks later, we shaved the hind limbs of the rats.









2.4.2Observed and compared the conditions of muscle growth among group A、B、



The rat of group A (Myostatin HMP)




Myostatin-300mcg every 3day-VS-blank：the Left one is from group A(Myostatin HMP), and the right one is from the negative control group.


2.5 Comparison after anatomizing



2.5.1Five weeks later, we anaesthetized the rats to death.









The left was from group A (Myostatin HMP), and the right is from Group B (Negative control)



Please contact us if you have any question regarding the experiments.


We are sometimes asked, what differentiates us from other companies, I think, one answer to this question will be: we not only test every batch of our products through HPLC, MS, SDS-Pages, but also design the experiment that demonstrates the high quality of the products.

Originally Posted by icecube789

nice,very professional.
any other Animal Cell Culture Report?

Cube????