Liquid Glutamine any signifigance? [Forum Archive]

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In liquid solutions, L-glutamine is unstable at 4°C or higher temperatures. The molecule dissociates and toxic ammonium is created as a waste product of that reaction. This limits the stability of cell culture media; as a consequence, stock solutions of L-glutamine or solutions containing L-glutamine should be stored frozen. In addition, cell culture media should be changed frequently to ensure availability of this amino acid.

Dipeptides containing L-glutamine are extremely well suited to replace L-glutamine. They are stable over long periods of time, even when stored at room temperature. Cells can retrieve L-glutamine by cleaving the peptide bond. PromoCell offers several media with stable L-glutamine in the form of N-acetyl-L-Ala-L-Glu. The amount of available L-glutamine corresponds to that of L-glutamine normally present in the equivalent medium after the addition of the appropriate amount of L-glutamine.