I like to make it up 5000 iu in 2 ml. That comes out to 0.1 ml per 250 iu. That's the dose I like to use EOD or ETD. If I have a 10000 iu vial I would use 4 ml. I would also use sterile saline 0.9%. It keeps the protein stable longer. Also in the future I would not purchase 10000 iu. I would get the 5000 iu vial b/c it goes bad to fast to use the 10000 iu size unless you do like 500 iu EOD. It lasts for 4-5 weeks in the refrigerator.