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How to sterilise vials and glassware?

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  1. #31
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    If you're using an oven you do not need to use rubbing alcohol, you just need to make sure there's no particulate in them.

    Rinse them out with hot water and Glass Magic or something of the sort, then bake. There will be absolutely no residue on the glass.

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    Quote Originally Posted by UA_Iron View Post
    If you're using an oven you do not need to use rubbing alcohol, you just need to make sure there's no particulate in them.

    Rinse them out with hot water and Glass Magic or something of the sort, then bake. There will be absolutely no residue on the glass.
    OK, thanks, I will try it.

    So you say using hot water and the Glass Magic or something like that will remove the particulates from inside of the vials ?

    I have to use the Glass Magic after the hot water, or together ?

  3. #33
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    how much are vials? and how much is your life worth?


  4. #34
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    Quote Originally Posted by probuilder View Post
    OK, thanks, I will try it.

    So you say using hot water and the Glass Magic or something like that will remove the particulates from inside of the vials ?

    I have to use the Glass Magic after the hot water, or together ?
    Use the dishwasher liquid that leaves glass spotless - have that in solution with hot water - dunk the vials in them. Rinse them with regular hot water multiple times, then the final solution rinse should be distilled water.

    Then heat sterilize.

    The rubbing alcohol is leaving residue on the glass its because its not fully cleaning it and/or it's leaving a residue behind (which i sure as F dont wanna inject).

    A proper sanitation rinse is probably better than rubbing alcohol as far as residue is concerned. Either way, the dry heat sterilization will work fine for this regardless of the alcohol being applied before baking or not.

  5. #35
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    Alcohol bath. Then right in a convention oven @ 250 degrees for 20 min. Turn oven off, remove with rubber gloves from oven AS you need them. Your good to go.

    Can microwave them for a few min if you want to.


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  6. #36
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    I do mine like this:

    Wash all parts in anti-bacterial soap and hot water (I use a baby bottle nipple cleaner with stainless steel brush tip to clean the inside of all bottles), rinse with hot water, from here they go into 50% alch solution, from here onto a pan with aluminum foil top down.
    Place in oven at 350 for 30 minutes.

    Rubber stoppers get done this way:
    Wash in anti-bacterial soap, rinsed in hot water, sit in alch solution, placed face UP(this means the part of the stopper that goes inside the vial is face up)

    Build brew, take vials out of oven, let cool to the touch while you finish brew.
    Move vials from pan to counter, vilter into vial. stopper as soon as the vial is at the apropriate level.
    Cap and your done.

    There is no need to bake a second time, as long as your workspace is clean (i like to use anti-bacterial wipes on everything all the time) you should be fine.
    I have made and ran 50 weeks of product this way with not a single issue ever.
    2% ba, 18%bb

    I also do all my lab ware the very same way.
    Media bottles can be done this way, but its much more difficult to clean the inside, IMO best to use single use bottles unless your equiped to clean the inside of hte bent neck ones.

    I have never seen a residue from the alch that I use, Pickup at drug store, its standard rubbing alch that you can get at any drugstore, pharmacy, costco, food store etc.

    THIS IS AN INCORECT STATEMENT.
    8. Add BA to your oil, this keeps the inside of the vial sterile yet again.

    BA only prevents the bacteria from growing INSIDE the vial, if its already in there your too late. Once this bacteria gets into the body it now has a nice warm moist enviroment to grow and cause potential issues. BA is an inhibitor not a killer.

    Keep the brewing questions posting!

  7. #37
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    Thanks for all of you !!

    Darkrid3r, just one question about your brewing : you wrote after the vials have been sterilized and finished the brew, filter, fill it and cap it.

    Shouldn't the vials be capped right after sterilizing ? I mean it can be contaminated by the air or the environment.

    Of course this way you do is much more casual, filling the vials like this. Above all at bigger amounts, like from a 500 or 1.000 ml media bottles

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