Sterilization Methods

Results 1 to 17 of 17
  1. #1
    Registered User


    Join Date
    Oct 2011
    Gender
    Male
    Location
    Mars
    Posts
    205
    Rep Points
    7238470

    Sterilization Methods






    Oven
    Dry heat is less effective than steam for sanitizing and sterilizing, but many brewers use it. The best place to do dry heat sterilization is in your oven. To sterilize an item, refer to the following table for temperatures and times required.

    Table 3 - Dry Heat Sterilization

    Temperature Duration

    338°F (170°C) 60 minutes

    320°F (160°C) 120 minutes

    302°F (150°C) 150 minutes

    284°F (140°C) 180 minutes

    250°F (121°C) 12 hours (Overnight)

    The times indicated begin when the item has reached the indicated temperature. Although the durations seem long, remember this process kills all microorganisms, not just most as in sanitizing. To be sterilized, items need to be heat-proof at the given temperatures. Glass and metal items are prime candidates for heat sterilization.

    Some homebrewers bake their bottles using this method and thus always have a supply of clean sterile bottles. The opening of the bottle can be covered with a piece of aluminum foil prior to heating to prevent contamination after cooling and during storage. They will remain sterile indefinitely if kept wrapped.

    One note of caution: bottles made of soda lime glass are much more susceptible to thermal shock and breakage than those made of borosilicate glass and should be heated and cooled slowly (e.g. 5 °F per minute). You can assume all beer bottles are made of soda lime glass and that any glassware that says Pyrex or Kimax is made of borosilicate.


    Autoclaves and Pressure Cookers

    Typically when we talk about using steam we are referring to the use of an autoclave or pressure cooker. These devices use steam under pressure to sterilize items. Because steam conducts heat more efficiently, the cycle time for such devices is much shorter than when using dry heat. The typical amount of time it takes to sterilize a piece of equipment in an autoclave or pressure cooker is 20 minutes at 257° F (125 °C) at 20 pounds per square inch (psi).

    BUY STERILE SEALED VIALS
    You can also buy sealed vials and remove the aluminum cap and then use a syringe needle to vent the stopper and fill will a syringe with a 18 or 15g needle Then recap. This is more expensive to do but if you are only doing it at a small scale then this maybe best for you.

    Please Vet brewers add Ideas to this thread to help noobs keep there glass vials sterile.

  2. #2
    Senior Member
    BOARD REP
    overburdened's Avatar


    Join Date
    Mar 2012
    Gender
    Male
    Location
    POLAND
    Posts
    1,402
    Rep Points
    47660934

    thanks for posting this, I'm sure some will find it informative... however... I do hope to god nobody is using beer bottles for brewing gear...

  3. #3
    Registered User


    Join Date
    Jul 2009
    Gender
    Male
    Location
    state of confusion and denial
    Posts
    3,263
    Rep Points
    98443740

    Beer bottles...crap I'm in the wrong homebrew section.

    Good read. I would rally recommend people buy a decent pressure cooker. Some good videos on YouTube.
    Please do not PM me with questions, I will not PM you back.

  4. #4
    BECAUSE I LOVE THIS SHIT!


    Join Date
    Feb 2011
    Gender
    Male
    Location
    Gearington U.S.A
    Posts
    695
    Rep Points
    15017040

    Quote Originally Posted by suprfast View Post
    Beer bottles...crap I'm in the wrong homebrew section.

    Good read. I would rally recommend people buy a decent pressure cooker. Some good videos on YouTube.
    I would but shit dry heat sterilization seems so much easier.

  5. #5
    Registered User


    Join Date
    Oct 2011
    Gender
    Male
    Location
    Mars
    Posts
    205
    Rep Points
    7238470

    Quote Originally Posted by overburdened View Post
    thanks for posting this, I'm sure some will find it informative... however... I do hope to god nobody is using beer bottles for brewing gear...
    LOL yeah that is just and example of the wrong type of glass buy the borosilicate vials cost a little more but thicker. Example
    10ml moulded vial | Med Lab Supply

  6. #6
    ♥ ♥ ♥ ♥ ♥ ♥
    BOARD REP
    Miss Springsteen's Avatar


    Join Date
    Dec 2011
    Gender
    Female
    Location
    Posts
    1,439
    Rep Points
    205294702

    Great read! Thanks!

    Do you want 15% off your next IronMagLabs order? use coupon code Miss15

    CHECK OUT MY ROAD TO DOMINATION
    Moderator at Fitnessgeared

  7. #7
    BECAUSE I LOVE THIS SHIT!


    Join Date
    Feb 2011
    Gender
    Male
    Location
    Gearington U.S.A
    Posts
    695
    Rep Points
    15017040

    Quote Originally Posted by towing View Post
    LOL yeah that is just and example of the wrong type of glass buy the borosilicate vials cost a little more but thicker. Example
    10ml moulded vial | Med Lab Supply
    So can I dry heat sterilize some vials that I have but will probably want to reuse. They are pre sterilized vials I got from med lab supply so hopefully they are thick enough.

  8. #8
    Registered User
    colochine's Avatar


    Join Date
    Aug 2011
    Gender
    Male
    Location
    florida
    Posts
    3,457
    Rep Points
    467341520

    I autoclave errything... No play no games here.

  9. #9
    Registered User


    Join Date
    Jul 2009
    Gender
    Male
    Location
    state of confusion and denial
    Posts
    3,263
    Rep Points
    98443740

    Quote Originally Posted by colochine View Post
    I autoclave errything... No play no games here.
    Please do not PM me with questions, I will not PM you back.

  10. #10
    Registered User


    Join Date
    Oct 2011
    Gender
    Male
    Location
    Mars
    Posts
    205
    Rep Points
    7238470

    If you clean out the old vials then wipe them with a sanitizing wipe then Dry heat you probably will be fine but yes the autoclave is the best way.

  11. #11
    BECAUSE I LOVE THIS SHIT!


    Join Date
    Feb 2011
    Gender
    Male
    Location
    Gearington U.S.A
    Posts
    695
    Rep Points
    15017040

    I don't have 5 bills to spend on a fancy pressure cooker lol I'll just buy a pressure cooker....

  12. #12
    Registered User


    Join Date
    Jul 2009
    Gender
    Male
    Location
    state of confusion and denial
    Posts
    3,263
    Rep Points
    98443740

    Quote Originally Posted by hill450 View Post
    I don't have 5 bills to spend on a fancy pressure cooker lol I'll just buy a pressure cooker....
    200 will get you a bullet proof pressure cooker that will last a lifetime. Less than 100 will get you a decent one that should last quite some time too.
    Please do not PM me with questions, I will not PM you back.

  13. #13
    Registered User


    Join Date
    Mar 2011
    Gender
    Male
    Location
    Awesome
    Posts
    302
    Rep Points
    19504397

    Unless you do a weekly spore test of your autoclave you have not a clue of its performance. And in fact the excess water can be a pathway for certain microorganisms to penetrate wet instrument packages.

    Dry heat is a very viable solution for glass items, and it is not less effective.

    Methodology and understanding of your tools is the biggest thing here.

  14. #14
    Registered User


    Join Date
    Sep 2011
    Gender
    Male
    Location
    Cali
    Posts
    58
    Rep Points
    1697576

    You guys think these will work ok? I know its prob not the best but for 5 bucks...

    Medela Quick Clean Micro-Steam Bags - 5ct - Medela - Babies "R" Us

  15. #15
    Senior Member
    BOARD REP
    BBPowder's Avatar


    Join Date
    Aug 2012
    Gender
    Male
    Location
    USA
    Posts
    700
    Rep Points
    11159367

    Sterilization Methods

    Oven works just fine. I dunk my vials in alcohol then put them in a convention oven at 450 for an hour or so.

    I fill the vials when they are still rather warm to the touch.

    I think a lot of people over think shit and freak out about this stuff.

    Remember, the first thing you do when you use AAS? You fill the syringe with air and push it into the vial to make positive pressure. So the first thing you do is un-sterialize your gear, then you use it.


    Ask for a product and price list!

  16. #16
    Registered User
    FordFan's Avatar


    Join Date
    Jul 2011
    Gender
    Male
    Location
    US
    Posts
    752
    Rep Points
    18063921

    Quote Originally Posted by BBPowder View Post

    Remember, the first thing you do when you use AAS? You fill the syringe with air and push it into the vial to make positive pressure. So the first thing you do is un-sterialize your gear, then you use it.
    Exactly!! However, that doesn't mean to not be clean during the process.
    Tren...it's what's for dinner!

  17. #17
    Senior Member
    BOARD REP
    BBPowder's Avatar


    Join Date
    Aug 2012
    Gender
    Male
    Location
    USA
    Posts
    700
    Rep Points
    11159367

    Sterilization Methods






    Quote Originally Posted by FordFan View Post
    Exactly!! However, that doesn't mean to not be clean during the process.
    Correct sir!

    Just keep things simple guys.


    Ask for a product and price list!

Similar Threads

  1. Sterilization techniques for AAS
    By TGB1987 in forum Anabolic Zone
    Replies: 16
    Last Post: 07-25-2011, 01:07 PM
  2. Sterilization
    By dcher002 in forum Anabolic Zone
    Replies: 13
    Last Post: 03-10-2011, 04:17 PM
  3. Replies: 62
    Last Post: 07-24-2010, 03:30 PM
  4. Sterilization...
    By RexLozano in forum Anabolic Zone
    Replies: 5
    Last Post: 08-28-2006, 01:25 PM

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
DISABLED END -->