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    Lightbulb Tired of all the sponsors that claim "Receptor Grade", "99.xx Purity"? Get Inside!






    Tired of all the sponsors that claim "Receptor Grade", "99.xx Purity" and a whole host of other useless claims?


    This is meant to be strictly educational so you guys understand what you are looking at and dispel some myths and mis-information.



    HPLC:

    This is a method where a mixture of compounds are studied and analyzed and broken down to see how many different components it is made up of. This methoddoes not identify the specific compound in hand at all. It does not give you the mass of the compound in daltons for you to cross match with what you are looking for. If you already have proof that you have something in that mixture it will show you purity but or not if there are several compounds are there. For example you can do an HPLC on a perfume to see how many different actual perfumes it is made up of etc. But for our purpose HPLC is pretty useless because it does not tell you anything useful. So when you see a sponsor post an HPLC of a so called product you need to be ware.

    This is what a typical HPLC looks like. Notice the X axis is always in time and not weight so it does not tell you anything. The Y axis is the concentration but then again does not identify the product.




    SDS-PAGE:

    This is a sodium gel electrophoresis and used heavily in genetics, forensics and chemistry where proteins are separated based on their ELECTROPHORETIC mobility which is a function of their polypeptide chain length and its electric charge. This shows up as a band on the gel. This is pretty decent in identifying if a certain protein exists at a certain weight range we are looking for. For example if we know that IGF LR3 has a 9111 dalton weight we should expect a thick heavy band in the 9000 range. The major issues with this procedure is that it is very limited and not very accurate down to the dalton weight. You may see a band in the ~9000 range but you dont know if it is 8800 or 9200 etc. And this is a huge issue when you are actually identifying a specific product. It also DOES NOT tell you how much total of a compound you have to begin with.


    This what a typical SDS-PAGE looks like. You can see bands showing up but there is nothing specific or accurate about it. For example, look at column 2. We can see a thick band showing up between 50-75kda but where does it actually fall? It is 58K or 63K? Who the hell knows. You also see lighter shadows around the thick band but doesnt tell you how bad the other impurities are.




    MASS SPEC:

    Mass spectrometry is where singular spectrum of the masses of the molecules comprising a sample of material are displayed. It really doesnt get much more accurate than this. It is basically used to determine the elemental composition of a sample based on the molecules mass weight. This will tell you not only if you have a pure sample but what the actual mass weight of the sample is and if there are other molecules that weight more or less than what you are looking for. Once again this will not tell you what is the total weight of your sample (as in total product weight). What i mean is that if i turn over a vial of IGF LR3 to the lab and they do a mass spec and it comes back with a perfect spike at 9111 daltons. All it means is that there was LR3 in there. It wont tell me if there was 0.1mg or 1mg or 10mg of LR3 in that sample. Hope that makes sense.

    This is what a typical MALDI TOF mass spec looks like. You can see peaks at the different exact mass where the compounds were detected. There is no guess work.





    So we have discussed the potential ways to identify a compound with a certain mass weight and discussed how mass spectrometry is the best method. But none of the above have verified what we actually have short of what is the mass weight of the molecule. Let me explain further. Since we have discussed IGF LR3 we will just stick with that. We know it has a mass weight of 9111 daltons. I can piece meal together enough crap protein in a chain in a lab to give you A SAMPLE that has a mass spec that will show up in the 9100 range. But does that mean just because you have a sample that weights close to 9111 that it is actually IGF LR3? HELL NO.

    The only way is to do a full amino acid analysis. There is a two fold reason for this. One it will show if the percentage of amnio acids found in the sample match the % of amino acids that SHOULD be in the LR3. Because we know from the sequence how many glutamaine, leucine, aspartamate etc are in one chain of LR3. So when you do an amino acid analysis those percentages should match up. For example, if your sample shows up with an amino acid that doesnt even exist in the LR3 chain you know something is screwy.

    The second reason for the amino acid analysis is that it will tell you within 10micrograms what the total weight of the protein you are testing for in that vial. What is the point of having a pure IGF LR3 but it really is 0.1mg vs 1mg or 10mg.

    AMINO ACID ANALYSIS NOT WILL CONFIRM AND BACK UP WHAT YOU FOUND IN THE MASS SPEC BUT ALSO TELL YOU THE TOTAL WEIGHT OF PRODUCT IN GIVEN SAMPLE.



    This is what a typical amino acid analysis looks like. I have taken out the name of the sponsor because this is not meant to be an advertisement and strictly educational. But you can see that we have the different amino acids listed on the left and what the known percentages should be vs what is the calculated from the sample. There is always a deviation allowed up to 1.0-1.5% considered very acceptable. You can see in this report that at the bottom there was a total of 952micrograms of LR3 found which is 0.952mg that is as close as to the 1.0mg advertised on the vial as you can get. You can also see that there was a slight amount of Histidine found in this sample. If you look at the sequence of IGF LR3 there is no Histidine found anywhere so to find it means that a very small sample had Histidine attached that were not cleaved off during the purification process. This is why amino acid analysis is so important.






    Conclusion:

    So what do we learn from all the above? No to be a sheep and just swallow everything that is fed to you. The only way any sponsor can prove to you what they are selling as far as a peptide/protein of any sort is to show you a proper Mass Spec report WITH a full amino acid analysis. One without the other is worthless. Just showing you a mass spec is worthless. Showing you HPLC reports and SDS PAGE reports shows that they really have no idea what they are doing. Please pass this information on as this should be a must read for everyone researching. Good luck

    I would like to give a special Thanks to Alpha for supplying us with this information!

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  2. #2
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    How many times you gonna post this bro? I haven't been here in a while and the first thing i see is this..which was just posted last time i was here...sheesh...get some new material or something!

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    Quote Originally Posted by lifterjaydawg View Post
    How many times you gonna post this bro? I haven't been here in a while and the first thing i see is this..which was just posted last time i was here...sheesh...get some new material or something!
    This Topic has been posted 1 time ~ a month ago.
    I was recently informed that the Topic had accidentally been deleted and I was asked to Re-post it.

    New Material added since Original Post Date:

    Hexarelin: In Depth Analysis | All About Peptides
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    Does it makes sense to run HGH, IGF1-LR3, GHRPs & GHRH simultaneously? | All About Peptides
    Do IGF and HGH cause bone and organ enlargement? can peptides replace them? | All About Peptides

    We thrive to give the community USEFUL and INFORMATIVE information, so if you have nothing positive to add, lets get .
    -AAP
    Last edited by AllAboutPeptides; 04-11-2013 at 02:26 PM.
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    For what it's worth. I have been around awhile and have not seen aap post this

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    What would you recommend to a guy... i mean lab rat...that is going to set up a 6-8 week cruise. My rat was thinking about some igf. The rat is coming of a 2g cycle and is looking to spice up his protocol a little. This rat has experience in ghrp 6 and 2, as well as cjc1295 and igf lr3. This rats going to eat clean for 6-8 weeks so the ghrp are out. Then he is going towards test/mast cycle so he really wants to be set up for being what they call around here "jerked and tan".

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    Quote Originally Posted by dieseljimmy View Post
    What would you recommend to a guy... i mean lab rat...that is going to set up a 6-8 week cruise. My rat was thinking about some igf. The rat is coming of a 2g cycle and is looking to spice up his protocol a little. This rat has experience in ghrp 6 and 2, as well as cjc1295 and igf lr3. This rats going to eat clean for 6-8 weeks so the ghrp are out. Then he is going towards test/mast cycle so he really wants to be set up for being what they call around here "jerked and tan".
    You've got the right idea!
    Igf Lr3 will stimulate cell division, known as mitogenesis, and cell differentiation. Meaning That In the case of IGF1 which does act on muscle tissue, will initiate the growth of new muscle fibers, and subsequently new receptors for testosterone. Many Users here and on other forums have unanimously concluded that it enhances cycles of steroids significantly. They also seem to be adamant about its ability to reduce fat and improve vascularity a great deal.
    Also, IGF-1 because of its action of forcing nutients into the muscle, increases the uptake of amino acids!
    Lr3 would be a great addition Imo.
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    Get the real deal !
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    i am running hgh and peps at the same time and i am losing fat and gaining muscle at the same time which is great. but i am all so bloated . should i drop the peps while on hgh and save the peps for when i run out of hgh. by the way i am running cjc no dac and ipam and hgh dosing all at the same time like i say geting great results just bloated. just wondering if it is the peps that is bloating me or the hgh or both? and what do most people do to loose the bloat from hgh or pepes? thanks

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    i have noticed some slight bloat from cjc


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    think i am going to drop everything but hgh see if it is the peps or the hgh.

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    Many Users here and on other forums have unanimously concluded that it enhances cycles of steroids significantly. They also seem to be adamant about its ability to reduce fat and improve vascularity a great deal.
    Also, IGF-1 because of its action of forcing nutients into the muscle, increases the uptake of amino acids!
    Lr3 would be a great addition Imo. [/QUOTE

    Yes Yes Yes!!! To anybody who is going to run gears if you add IGF1LR3 and use it correctly meaning dont take it for at least 4hrs PW then the results are like nothinh you will ever get with just gear alone... Ive said it many times before... the results were nothing short of amazing in the short amount of time I used it... Unbelievable!!!

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