I do mine like this:
Wash all parts in anti-bacterial soap and hot water (I use a baby bottle nipple cleaner with stainless steel brush tip to clean the inside of all bottles), rinse with hot water, from here they go into 50% alch solution, from here onto a pan with aluminum foil top down.
Place in oven at 350 for 30 minutes.
Rubber stoppers get done this way:
Wash in anti-bacterial soap, rinsed in hot water, sit in alch solution, placed face UP(this means the part of the stopper that goes inside the vial is face up)
Build brew, take vials out of oven, let cool to the touch while you finish brew.
Move vials from pan to counter, vilter into vial. stopper as soon as the vial is at the apropriate level.
Cap and your done.
There is no need to bake a second time, as long as your workspace is clean (i like to use anti-bacterial wipes on everything all the time) you should be fine.
I have made and ran 50 weeks of product this way with not a single issue ever.
2% ba, 18%bb
I also do all my lab ware the very same way.
Media bottles can be done this way, but its much more difficult to clean the inside, IMO best to use single use bottles unless your equiped to clean the inside of hte bent neck ones.
I have never seen a residue from the alch that I use, Pickup at drug store, its standard rubbing alch that you can get at any drugstore, pharmacy, costco, food store etc.
THIS IS AN INCORECT STATEMENT.
8. Add BA to your oil, this keeps the inside of the vial sterile yet again.
BA only prevents the bacteria from growing INSIDE the vial, if its already in there your too late. Once this bacteria gets into the body it now has a nice warm moist enviroment to grow and cause potential issues. BA is an inhibitor not a killer.
Keep the brewing questions posting!
