yeah youre right. your estradiol is mid-range. thats what set me off. Because Ive ran just a clomid pct and had a lower est (12.4)so it just made me believe it didnt work.
this is good. I wanted to be proven wrong.
since your test is so low, have you thought about throwing in some DAA I found two great articles on it. Not to say you dont already know about it. I just wanted to share.
Here are two articles from the
US National Library of Medicine and
National Institutes of Health about D-Aspartic Acid.
The role and molecular mechanism of D... [Reprod Biol Endocrinol. 2009] - PubMed - NCBI
Reprod Biol Endocrinol. 2009 Oct 27;7:120. The role and molecular mechanism of D-aspartic acid in the release and synthesis of LH and testosterone in humans and rats. Topo E, Soricelli A, D'Aniello A, Ronsini S, D'Aniello G. Source
1Stazione Zoologica Anton Dohrn, 80121, Villa Comunale, 80121, Napoli, Italy.
enza.topo@szn.it
Abstract
BACKGROUND: D-aspartic acid is an amino acid present in neuroendocrine tissues of invertebrates and vertebrates, including rats and humans. Here we investigated the effect of this amino acid on the release of LH and testosterone in the serum of humans and rats. Furthermore, we investigated the role of D-aspartate in the synthesis of LH and testosterone in the pituitary and testes of rats, and the molecular mechanisms by which this amino acid triggers its action.
METHODS: For humans: A group of 23 men were given a daily dose of D-aspartate (DADAVIT) for 12 days, whereas another group of 20 men were given a placebo. For rats: A group of 10 rats drank a solution of either 20 mM D-aspartate or a placebo for 12 days. Then LH and testosterone accumulation was determined in the serum and D-aspartate accumulation in tissues. The effects of D-aspartate on the synthesis of LH and testosterone were gauged on isolated rat pituitary and Leydig cells. Tissues were incubated with D-aspartate, and then the concentration (synthesis) of LH and cGMP in the pituitary and of testosterone and cAMP in the Leydig cells was determined.
RESULTS: In humans and rats, sodium D-aspartate induces an enhancement of LH and testosterone release. In the rat pituitary, sodium D-aspartate increases the release and synthesis of LH through the involvement of cGMP as a second messenger, whereas in rat testis Leydig cells, it increases the synthesis and release of testosterone and cAMP is implicated as second messenger. In the pituitary and in testes D-Asp is synthesized by a D-aspartate racemase which convert L-Asp into D-Asp. The pituitary and testes possesses a high capacity to trapping circulating D-Asp from hexogen or endogen sources.
CONCLUSION: D-aspartic acid is a physiological amino acid occurring principally in the pituitary gland and testes and has a role in the regulation of the release and synthesis of LH and testosterone in humans and rats.
D-Aspartic acid: an endogenous amino acid with... [Brain Res Rev. 2007] - PubMed - NCBI
Brain Res Rev. 2007 Feb;53(2):215-34. Epub 2006 Nov 21. D-Aspartic acid: an endogenous amino acid with an important neuroendocrine role. D'Aniello A.
Source: Laboratory of Neurobiology, Stazione Zoologica A Dohrn, Villa Comunale 1, 80121 Napoli, Italy.
daniello@szn.it
Abstract
D-Aspartic acid (d-Asp), an endogenous amino acid present in vertebrates and invertebrates, plays an important role in the neuroendocrine system, as well as in the development of the nervous system. During the embryonic stage of birds and the early postnatal life of mammals, a transient high concentration of d-Asp takes place in the brain and in the retina. d-Asp also acts as a neurotransmitter/neuromodulator. Indeed, this amino acid has been detected in synaptosomes and in synaptic vesicles, where it is released after chemical (K(+) ion, ionomycin) or electric stimuli. Furthermore, d-Asp increases cAMP in neuronal cells and is transported from the synaptic clefts to presynaptic nerve cells through a specific transporter. In the endocrine system, instead, d-Asp is involved in the regulation of hormone synthesis and release. For example, in the rat hypothalamus, it enhances gonadotropin-releasing hormone (GnRH) release and induces oxytocin and vasopressin mRNA synthesis. In the pituitary gland, it stimulates the secretion of the following hormones: prolactin (PRL), luteinizing hormone (LH), and growth hormone (GH) In the testes, it is present in Leydig cells and is involved in testosterone and progesterone release. Thus, a hypothalamus-pituitary-gonads pathway, in which d-Asp is involved, has been formulated. In conclusion, the present work is a summary of previous and current research done on the role of d-Asp in the nervous and endocrine systems of invertebrates and vertebrates, including mammals.
PMID: 17118457 [PubMed - indexed for MEDLINE]
(<<<if you couldn't tell, that was full homo)
