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my DNP cycle
- Thread starter Nachez
- Start date
lmao!outofshapecis said:
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Are you preparing for a show? How long do you intend to use DNP?
See, here's the thing we need to talk about, you and me. Tight junctions in certain tissues and the importance of their integrity.
Anywhere you got ions pumped in and out of special structures in cells that are energy dependent, you've probably got very tightly packed arrange of cellular junctions, called tight junctions. They allow for a gradient, a differnce in ion concentration to exist across a membrane, just as filter might control the movement of particles between one space and another.
Mostly, we're taking about important regulators, like calcium, magnesium, and sodium and potassium.
These tight junctions, they're critical to function of your kidneys, your heart and muscle tissue, they're found at the base of your brain, gating the flow of ions and compounds between your blood and the fluid inside your brain.
Now DNP at low levels, it fucks up the action of ATP (energy) regulation of these ion channels that are important to the function of these tight junctions.
At higher concentrations of dinitrophenol, a common lab solvent (by the way) that is lipophilic - it perferentially concentrates in membranes - will disrupt the function of these ion channel, and can irreversibly damage these membranes.
Maybe not such a good idea.
At low levels, it distrupts the smooth action of muscle contraction. Thats important to bodybuilders, you know. You're already seeing kidney effects. Now, you continue to use caffeine and other dehyration agents, and that will put further stress on your kidneys. You don't want them to stop doing their thing, controlling the oh so delicate balance of potassium and sodium. Little too much or too little - calcium, sodium, potassium - and you can screw up another muscle - the heart.
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Myosin II-actin interaction in MDCK cells: role in cell shape changes in response to Ca2+ variations. Castillo AM, Lagunes R, Urban M, Frixione E, Meza I.
Cultured MDCK cell monolayers respond to a low level of extracellular calcium ([Ca2+]e < or = 5 microM) with a loss of transepithelial electrical resistance and transport function, and changes in position of a circumferential ring of actin filaments tethered to the plasma membrane at the zonula adhaerens. Keeping this cytoskeletal structure in place seems necessary to preserve the architecture of the tight junctions and therefore their sealing capacity. All three effects are reversible upon restituting normal [Ca2+]e. Recent work provided evidence of actin-myosin interactions at the filament ring, thus suggesting a contraction process involved in the alteration of the actin cytoskeleton. We now report that active contraction does occur and causes an extensive morphological transformation of MDCK cells. A marked increase in cell height simultaneous with a decrease in width and area of contact to the substratum was seen within 10 min of removal of [Ca2+]e; recovery began immediately after replacing calcium, although it took longer for completion. Conventional and confocal epifluorescence studies showed actin colocalized with myosin II at various planes of resting or contracted cells, in particular at the ring level. Electron-micrographs revealed the circumferential actin ring associated with the plasma membrane in a waist-like constriction where Ca2+ was removed from the cultures.
---> Contraction, as well as relaxation, in response to [Ca2+]e variations were inhibited by cytochalasin-D (an actin-filament disrupting drug), by okadaic acid( an inhibitor of myosin light-chain dephosphorylation), and by 2,3-butanedione monoxime (a blocker of myosin II ATPase activity).
---> Similarly, no response [no contraction, and no relaxation] was observed in cells previously depleted of metabolic energy by *2,4-dinitrophenol* and 2-deoxy-D-glucose preincubation. The actin-myosin mediated reversible structural transformation of MDCK cells in response to [Ca2+]3 poses new questions for the interpretation of in vitro experiments, as well as for the understanding of epithelial function.
MDCK cells are an easily testable muscle cell model system.
Take home message: You only use DNP for short periods. No more than 10 days, a week is even better. You should only be using it for removing the very last few pounds, just a couple, before a bodybuilding show or competition. You weigh the benefits and risks of use of DNP. Its potent, it bioaccumulates in membranes, and it can have low level effects that you might not notice, but they're there, and they're a measurable cost for its use.
That's why some folks who commented here mention that other methods for fat loss should be used before this more drastic and costly chemical is employed. Its not for spring cutting, for the beaches.
This commentary isn't meant to be judgemental. Its just a notation, and a bit of warning. Got to know what you're using, and balance why, the benefits - sensibly against the risks.
See, here's the thing we need to talk about, you and me. Tight junctions in certain tissues and the importance of their integrity.
Anywhere you got ions pumped in and out of special structures in cells that are energy dependent, you've probably got very tightly packed arrange of cellular junctions, called tight junctions. They allow for a gradient, a differnce in ion concentration to exist across a membrane, just as filter might control the movement of particles between one space and another.
Mostly, we're taking about important regulators, like calcium, magnesium, and sodium and potassium.
These tight junctions, they're critical to function of your kidneys, your heart and muscle tissue, they're found at the base of your brain, gating the flow of ions and compounds between your blood and the fluid inside your brain.
Now DNP at low levels, it fucks up the action of ATP (energy) regulation of these ion channels that are important to the function of these tight junctions.
At higher concentrations of dinitrophenol, a common lab solvent (by the way) that is lipophilic - it perferentially concentrates in membranes - will disrupt the function of these ion channel, and can irreversibly damage these membranes.
Maybe not such a good idea.
At low levels, it distrupts the smooth action of muscle contraction. Thats important to bodybuilders, you know. You're already seeing kidney effects. Now, you continue to use caffeine and other dehyration agents, and that will put further stress on your kidneys. You don't want them to stop doing their thing, controlling the oh so delicate balance of potassium and sodium. Little too much or too little - calcium, sodium, potassium - and you can screw up another muscle - the heart.
-----------------------------------------------------------------------
Myosin II-actin interaction in MDCK cells: role in cell shape changes in response to Ca2+ variations. Castillo AM, Lagunes R, Urban M, Frixione E, Meza I.
Cultured MDCK cell monolayers respond to a low level of extracellular calcium ([Ca2+]e < or = 5 microM) with a loss of transepithelial electrical resistance and transport function, and changes in position of a circumferential ring of actin filaments tethered to the plasma membrane at the zonula adhaerens. Keeping this cytoskeletal structure in place seems necessary to preserve the architecture of the tight junctions and therefore their sealing capacity. All three effects are reversible upon restituting normal [Ca2+]e. Recent work provided evidence of actin-myosin interactions at the filament ring, thus suggesting a contraction process involved in the alteration of the actin cytoskeleton. We now report that active contraction does occur and causes an extensive morphological transformation of MDCK cells. A marked increase in cell height simultaneous with a decrease in width and area of contact to the substratum was seen within 10 min of removal of [Ca2+]e; recovery began immediately after replacing calcium, although it took longer for completion. Conventional and confocal epifluorescence studies showed actin colocalized with myosin II at various planes of resting or contracted cells, in particular at the ring level. Electron-micrographs revealed the circumferential actin ring associated with the plasma membrane in a waist-like constriction where Ca2+ was removed from the cultures.
---> Contraction, as well as relaxation, in response to [Ca2+]e variations were inhibited by cytochalasin-D (an actin-filament disrupting drug), by okadaic acid( an inhibitor of myosin light-chain dephosphorylation), and by 2,3-butanedione monoxime (a blocker of myosin II ATPase activity).
---> Similarly, no response [no contraction, and no relaxation] was observed in cells previously depleted of metabolic energy by *2,4-dinitrophenol* and 2-deoxy-D-glucose preincubation. The actin-myosin mediated reversible structural transformation of MDCK cells in response to [Ca2+]3 poses new questions for the interpretation of in vitro experiments, as well as for the understanding of epithelial function.
MDCK cells are an easily testable muscle cell model system.
Take home message: You only use DNP for short periods. No more than 10 days, a week is even better. You should only be using it for removing the very last few pounds, just a couple, before a bodybuilding show or competition. You weigh the benefits and risks of use of DNP. Its potent, it bioaccumulates in membranes, and it can have low level effects that you might not notice, but they're there, and they're a measurable cost for its use.
That's why some folks who commented here mention that other methods for fat loss should be used before this more drastic and costly chemical is employed. Its not for spring cutting, for the beaches.
This commentary isn't meant to be judgemental. Its just a notation, and a bit of warning. Got to know what you're using, and balance why, the benefits - sensibly against the risks.
Yep, if your potassium gets too low, you set your self up for a possible heart attack!
Are you certified at all? If you want to look like that you could always consider becoming a RD.
When you use drugs to get to a certain goal, if you dont have the means to maintain that (knowledge/discipline) then you are going to rebound anyway.
When you use drugs to get to a certain goal, if you dont have the means to maintain that (knowledge/discipline) then you are going to rebound anyway.
You think that came from DNP?Nachez said:
preparing yourself for personal training by running DNP? mind explaining the link to me between DNP and personal training?Nachez said:
Personal training is about knowledge not looks son, the only trainer I payed for was 5'11 and about 155...he couldn't even bench 200lbs. But the guy was a fucking expert on every topic discussed on this site.Nachez said:
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The only guy I've ever talked to who could actually go into deep detail on every subject I've seen on this site, in magazines, or studies was like 5'9 with a big gut. Even more so he was a salesman, but more then that he was just a general fitness guru ( as it seemed ), he was even extremely knowledgeable about random little known organic remedies and supplements. I later found out he'd been in the fitness game for 25+ years. He was part of the recon unit of the Marines in Vietnam, and more so he was friendly. Most of the guys I see at gyms ( I don't use them generally, as I prefer my schools weightroom ) are just idiots even though they have the genetics.
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well I am very knowledgable on all kinds of things, my problem is a slow metabolism
thats why i ran the DNP cycle.
so id get cut quick, so i could attract clientel.
I have no problem gaining muscle
just have a problem losing fat
have fat genes on both sides of my family.
wasnt genetically blessed like black and asian dudes
who are naturally low body fat, and cut with little effort. Wasnt blessed with the high metabolism.
thats why i ran the DNP cycle.
so id get cut quick, so i could attract clientel.
I have no problem gaining muscle
just have a problem losing fat
have fat genes on both sides of my family.
wasnt genetically blessed like black and asian dudes
who are naturally low body fat, and cut with little effort. Wasnt blessed with the high metabolism.
Is this sarcasm? RDs always seem to get a bad rap on bb boards. I've been in an RD program for over two years, and I can't imagine a better education that would enable someone to achieve their optimal physique through diet and exercise. We don't focus much on kinesiology, but the biochemistry, anatomy, and physiology one must learn to become an RD is amazing. You learn every principle there is concerning the regulation of energy metabolism, lipolysis, anabolism, etc. RDs deserve much more respect than they usually get on the boards. Their knowledge and expertise can be applied to bodybuilders just as well as the average pie eater.Mudge said:
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Nachez said:
*rolling eyes*
Pirate! said:
I never knew they had a bad rap. If you mean via kiddie posters or at places where George is "yours in sport," then I can imagine the posts such places might display would be rather moronic.
Rd?
