Just got done with my Research and had to bake it in the oven as instructed @ 250F. I was supplied with an 18g needle to use for venting. Once it was finished baking I removed the needle and I could see that it had an opening about the size of the needle. The hole was mostly closed but when I turned it upside down, little bubbles were coming up through the oil. None of the oil was coming through.
Was this caused by me removing the needle before it was completely cooled off and then that created a vacuum within the vial itself and now it is just equalizing the pressure?? Should I be concerned about the sterility of my research project? I know we are suppose to shoot air from the syringe directly into the vial in order to create the pressure.
Has anyone experienced this and if so, what should I do about it? Bake it again?
Was this caused by me removing the needle before it was completely cooled off and then that created a vacuum within the vial itself and now it is just equalizing the pressure?? Should I be concerned about the sterility of my research project? I know we are suppose to shoot air from the syringe directly into the vial in order to create the pressure.
Has anyone experienced this and if so, what should I do about it? Bake it again?




