How to sterilise vials and glassware?

[Dannie]

I am going to start homebrewing me own supplies soon

To Avoid infection I would like to know how to sterilise glassware, vials and rubber stoppers?
What chemicals should I use.

I have a pressure cooker I could use, if thats any better than oven

 

[Digitalash]

most people use pre-sterilized vials, they come in any size you need

otherwise you will need to buy a crimper which isn't exactly cheap and sterilize each piece yourself

 

[RAWS n More]

most people use pre-sterilized vials, they come in any size you need

otherwise you will need to buy a crimper which isn't exactly cheap and sterilize each piece yourself

I get it all sterile also. An autoclave is what most use, at least who i know.

 

[Digitalash]

yeah I guess a pressure cooker might work but it's not optimal. Unless you have an autoclave and a crimper you're probably better off getting prestilized lol. Unless you're starting your own lab or something you won't need to get that serious. Brew all your gear in one large vial, and then draw it out into 10ml's if you like.

 

[keith1569]

Why risk it. Just buy presterile vials

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[bulldogz]

Too much work to sterilize vials yourself...just buy them already sterile

 

[PurePersian]

I am going to start homebrewing me own supplies soon

To Avoid infection I would like to know how to sterilise glassware, vials and rubber stoppers?
What chemicals should I use.

I have a pressure cooker I could use, if thats any better than oven

Auto clave is the pro way but without one
This is One way is to
Step one: Submerse the vials in alcohol and pre heat (350)oven pull vials out of alchohol. Let dry on sterile surface.
Step Two: (INSERT Vitamin Liquid)... This Step allows the liquid to be readily in vials for stopper
Step Three: Place into 350 deg oven for upto thirty minutes(some people do 400 one hour!!?!) Temp can Vary depending on what your cooking! You DONT ever want to over cook! .. Watch out for boil over!! Do Not Over Load Oven With Too Many!
Step Four: Submerge stoppers in Hydrogen Perioxide or you can use Alcohol...( I used perioxide.. some of my buddies use alcohol)
Step Five: Once Cooking of vials is complete. Carefully and quickly put stopper onto vials once they are cooked crimp tops on with crimping tool while they are hot.
And Bam!! Your done bro!

Check other areas in the forum for more info on what your placing in your vials.. Vitamin b Right??

This is how it in the past and it went smooth, but if anyone cares to to correct anything feel free! Pro brewers feel Free to Chime in.

In no way is this information intended for or to be used in or as any legal medical sterilization techniques or be used to facilitate any illegal intentions.
These are all just my opinions.

Good Luck Bro!

 

[lovethislife]

Auto clave is the pro way but without one
This is One way is to
Step one: Submerse the vials in alcohol and pre heat (350)oven pull vials out of alchohol. Let dry on sterile surface.
Step Two: (INSERT Vitamin Liquid)... This Step allows the liquid to be readily in vials for stopper
Step Three: Place into 350 deg oven for upto thirty minutes(some people do 400 one hour!!?!) Temp can Vary depending on what your cooking! You DONT ever want to over cook! .. Watch out for boil over!! Do Not Over Load Oven With Too Many!
Step Four: Submerge stoppers in Hydrogen Perioxide or you can use Alcohol...( I used perioxide.. some of my buddies use alcohol)
Step Five: Once Cooking of vials is complete. Carefully and quickly put stopper onto vials once they are cooked crimp tops on with crimping tool while they are hot.
And Bam!! Your done bro!

Check other areas in the forum for more info on what your placing in your vials.. Vitamin b Right??

This is how it in the past and it went smooth, but if anyone cares to to correct anything feel free! Pro brewers feel Free to Chime in.

In no way is this information intended for or to be used in or as any legal medical sterilization techniques or be used to facilitate any illegal intentions.
These are all just my opinions.

Good Luck Bro!

X2 good shit

 

[keith1569]

interesting bro..anyone else try that method yet?

 

[brundel]

Cover a new baking pan with aluminum foil.
Place your vials onto the pan.
Cover the tops individually with foil.
Bake at 350 for 1hr.

Done.

The vials will remain sterile for as long as they are sealed with foil.


Filter all your gear into 100ml vials....just get pre sterilized ones...they are like 30cents.
Once you have all your gear filtered.

Wear gloves and work in a clean environment.
Draw 10ml into a large syringe.
Puncture foil and fill vial.

FIll a small dish with BA
using large tweezers dip the stopper in BA.
remove the foil and push on stopper using tweezers.
Cap and crimp.

 

[PurePersian]

Ya don't forget foil over the tops! Nice post brundel..brundel way is dead on also fellas. Ive always used thirty minutes to an hour..depends from what Ive experienced either way gtg!

 

[keith1569]

great info! im definately considering this now..sounds fun in a way

if one was wanting to use the fliptops instead of just stoppers, would u still just dip them in BA or peroxide?

as for the foil on the empty vials, can i dip that in BA also before i cover the vials with it or is that overkill?

 

[PurePersian]

great info! im definately considering this now..sounds fun in a way

if one was wanting to use the fliptops instead of just stoppers, would u still just dip them in BA or peroxide?

as for the foil on the empty vials, can i dip that in BA also before i cover the vials with it or is that overkill?

Consider overkill a good thing...a minute is well worth no infection...yes that would work too your first question.

 

[keith1569]

Ya I prefer overkill ha.
Thanks for the answer.

Out of curiosity how many avails have you sterilized this way without issue?

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[PurePersian]

Out of curiosity how many avails have you sterilized this way without issue?

Im sure Brundel has done more than me bro as he is a guru on the boards lol..but a good amount bro... Think about powders... how much you buy..how many vials it makes...ex..12.5g=5 vials at 10ml..

 

[keith1569]

Ya I know what ya mean. I was just wondering if u had used the method posted pleanty of times without infection issues.
I'm drawn to be able to have the flip top on it! Ha

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[gymrat09091974]

im crimping my next batch of vials, just looks professional.

 

[keith1569]

Nice! When that gonna be? Will u post pics??

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[gymrat09091974]

might be a bit. i went a little crazy buying vials this time

 

[keith1569]

Haha ya I feel ya. Easy to do!

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[tyzero89]

I have a question about Brundels method...How do you prevent or remove any particles from the vials?? should you rinse them with alcohol before baking at 350 for an hour??

 

[Bigbully100678]

I haven't done it before, but I have been told to rinse them with distilled water to remove any particulates before sterilizing.

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[PurePersian]

Yes you can use alcohol (some people do hydrogen perioxide) IMO

 

[Thresh]

1. Alcolhol bath vials.
2. Let dry, technically they should be sterile as the alcohol evaporates.
3. Microwave on high for 5min. This will kill any remaining bacteria.
4. Place in convention oven upside down and cook for a little.
5. Make your roids.
6. Alcohol bath rubber stoppers , take vials out of oven.
7. Fill vials and cap them.
8. Add BA to your oil, this keeps the inside of the vial sterile yet again.

 

[keith1569]

I have a question about Brundels method...How do you prevent or remove any particles from the vials?? should you rinse them with alcohol before baking at 350 for an hour??

I would say wash them in distilled water first. Then give them a nice bath in alcohol.

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[UA_Iron]

An autoclave is a pressure cooker. Autoclaves might come with more features like blow off valves etc.

Anyone ever notice alcohol leaves a residue on their vials after using it?

 

[Gutterpunk]

I use to work at a tattoo shop and the health inspector said that the only legal alternative to a sterilizer is a presure cooker. That even if you boiled the stuff it wouldn't be hot enough to kill the germs. Hope this helps.

 

[colochine]

Autoclaves rock...

 

[UA_Iron]

I use to work at a tattoo shop and the health inspector said that the only legal alternative to a sterilizer is a presure cooker. That even if you boiled the stuff it wouldn't be hot enough to kill the germs. Hope this helps.

You need the pressure in there too to make it completely unlivable for any bacteria and virus.

 

[probuilder]

An autoclave is a pressure cooker. Autoclaves might come with more features like blow off valves etc.

Anyone ever notice alcohol leaves a residue on their vials after using it?

I have this problem now. I wanted to sterilize 10 ml vials and an 1.000 ml media bottle.
I rinsed them in alcohol, placed in oven to dry, but there has been residues in all of them.

Before it didn't happen. The problem is I think that I reuse the alcohol, and after a time it has polluted by the dust from the air, from the vials and large beaker.

How can I avoid this problem ?

I know - just buy presterilized vials and that's it.
But I want to use flip off caps...

Other thing is I want to use large media bottles which need to be sterilized.

So removing particulates is my main problem.